recombinant mouse igf ii  (R&D Systems)

Journal: bioRxiv

Article Title: A novel approach for reliable differentiation of lymphatic endothelial progenitor cells in vitro

doi: 10.1101/2025.09.23.677051

Figure Lengend Snippet: Characterization of lymphatic endothelial progenitor cells (LEPCs) shows correct differentiation by overexpression of lymphatic lineage specific markers. (A) RT-qPCR shows statistically significant overexpression of key lymphatic markers such as Pdpn , Vegfr3 , Lyve1 , Vegf-c and Igf2 . n=4. MSC, mesenchymal stem cells. Mean ± SD. (B) Median fluorescence intensity analysis of immunofluorescence staining images shows statistically significant differential expression of PDPN between MSCs and LEPCs. Scale bar: 100 µm. n=6. Statistical significance: * p -value<0.05; ** p -value<0.01. Mann-Whitney test.

Article Snippet: At passage 3, MSCs were transferred to new dishes using 0.25% Trypsin-EDTA, and seeded at 20.000 cells/cm with complete mouse endothelial cell medium (ECGM, M1168, Cell Biologics) supplemented with 50 ng/mL of VEGF-C (752-VC, RD Systems), CCBE1 (H00147372-P01, Abnova), angiopoietin-2 (7186-AN, Angiopoietin-2), IGF2 (792-MG, RD Systems), FGF2 (3339-FB, RD Systems) and angiopoietin-1 (9936-AN, RD Systems).

Techniques: Over Expression, Quantitative RT-PCR, Fluorescence, Immunofluorescence, Staining, Quantitative Proteomics, MANN-WHITNEY

Journal: Journal of integrative neuroscience

Article Title: The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period.

doi: 10.31083/j.jin2311208

Figure Lengend Snippet: Fig. 4. Expression of the IGF system in rats with CPP. (A) The pub-score ratings of each group of rat ovaries and representative ovarian sections. Scale bar = 100 µm. (B) The mRNA expression levels of GnRH, IGF1, IGF1R, IGF2, and IGF2R in the hypothalamus

Article Snippet: The ratio of cell culture medium is as follows: 10% fetal bovine serum (F8318, Sigma, St. Louis, MO, USA), 1% dual antibody (15140-122, Gibco, St. Louis, MO, USA), and 89% high glucose Dulbecco’s modified Eagle medium (DMEM) (C11995500BT, Gibco, St. Louis, MO, USA), cultured in an environment of 5%CO2 at 37 °C.When the cells reached approximately 80% confluence, the culture mediumwas replaced with DMEM, and the cells were treated with varying concentrations of IGF2 protein (792-MG, R&D Systems, Minneapolis, MN, USA) (0, 1, 10 ng/mL), for 4 hours.

Techniques: Expressing

Journal: Journal of integrative neuroscience

Article Title: The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period.

doi: 10.31083/j.jin2311208

Figure Lengend Snippet: Fig. 5. GnRH synthesis and secretion in GT1-7 cells treated with different concentrations of IGF2 for 4 hours. Data are presented

Article Snippet: The ratio of cell culture medium is as follows: 10% fetal bovine serum (F8318, Sigma, St. Louis, MO, USA), 1% dual antibody (15140-122, Gibco, St. Louis, MO, USA), and 89% high glucose Dulbecco’s modified Eagle medium (DMEM) (C11995500BT, Gibco, St. Louis, MO, USA), cultured in an environment of 5%CO2 at 37 °C.When the cells reached approximately 80% confluence, the culture mediumwas replaced with DMEM, and the cells were treated with varying concentrations of IGF2 protein (792-MG, R&D Systems, Minneapolis, MN, USA) (0, 1, 10 ng/mL), for 4 hours.

Techniques:

Journal: Journal of integrative neuroscience

Article Title: The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period.

doi: 10.31083/j.jin2311208

Figure Lengend Snippet: Fig. 6. The expression of GnRH of hypothalamus in mice injected intracerebroventricularly with IGF2. (A) The pre-experiment

Article Snippet: The ratio of cell culture medium is as follows: 10% fetal bovine serum (F8318, Sigma, St. Louis, MO, USA), 1% dual antibody (15140-122, Gibco, St. Louis, MO, USA), and 89% high glucose Dulbecco’s modified Eagle medium (DMEM) (C11995500BT, Gibco, St. Louis, MO, USA), cultured in an environment of 5%CO2 at 37 °C.When the cells reached approximately 80% confluence, the culture mediumwas replaced with DMEM, and the cells were treated with varying concentrations of IGF2 protein (792-MG, R&D Systems, Minneapolis, MN, USA) (0, 1, 10 ng/mL), for 4 hours.

Techniques: Expressing, Injection

Journal: Aging Cell

Article Title: Premature cognitive decline in a mouse model of tuberous sclerosis

doi: 10.1111/acel.14318

Figure Lengend Snippet: Hippocampal administration of recombinant IGF2 normalizes both memory consolidation and relative cfos expression in aged Tsc2 +/− mice. (a) Schematic overview of 7d NORT including IGF2 administration on day 2 within 2 h after second training phase. (b), 7d NORT approach of aged Tsc2 mutant mice 7 days after IGF2 injection showed a significant enhancement of memory consolidation measured as discrimination index compared to the PBS‐injected mutant mice (two tailed t test: P (baseline) = 5,89e‐9, n (WT) = 32, n ( Tsc2+/− ) = 34); p (PBS) = 0.0066, n (WT) = 13, n ( Tsc2 +/− ) = 11; p (IGF2) = 0.4433, n (WT) = 18, n ( Tsc2 +/− ) = 17. (c) Relative mRNA expression levels of cfos in the hippocampus of aged Tsc2 mutants after IGF2 administration compared to the control group. No significant difference in Tsc2 +/− mice compared to wildtype controls after IGF2 treatment (two‐tailed t test: p (PBS) = 0.0004, n (WT) = 4, n ( Tsc2 +/− ) = 7); p (IGF2) = 0.2329, n (WT) = 6, n ( Tsc2 +/− ) = 8. Values were normalized against Gapdh and are presented as mean ± SEM, * p < 0.05, *** p < 0.001. Quantification was performed using Excel.

Article Snippet: A single dose of recombinant mouse IGF2 protein (R&D Systems, #792‐MG) was stereotactically injected into the hippocampus of 8–10 months old Tsc2 +/− mice and wildtype littermates after the training phase 2 (day 2) of the 7 days NORT (Figure ).

Techniques: Recombinant, Expressing, Mutagenesis, Injection, Two Tailed Test, Control